Part:BBa_K4613508

YF1-Motif_58.16-GFP

This composite part contains BBa_J64997, BBa_K592016, BBa_B0053, BBa_K2277233, BBa_K4613453, and BBa_E0040, which was used as a control to minimize the effect of variables and motif 58.16.

Reference

1. Ranzani A T, Wehrmann M, Kaiser J, et al. Light-dependent control of bacterial expression at the mRNA level[J]. ACS Synthetic Biology, 2022, 11(10): 3482-3492.
2. Weber A M, Kaiser J, Ziegler T, et al. A blue light receptor that mediates RNA binding and translational regulation[J]. Nature chemical biology, 2019, 15(11): 1085-1092.
3. Dietler J, Gelfert R, Kaiser J, et al. Signal transduction in light-oxygen-voltage receptors lacking the active-site glutamine[J]. Nature Communications, 2022, 13(1): 2618.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 581
  Illegal NgoMIV site found at 653
  Illegal NgoMIV site found at 743
  Illegal NgoMIV site found at 761
  Illegal NgoMIV site found at 1273
  Illegal NgoMIV site found at 1566
  Illegal NgoMIV site found at 1660
  Illegal AgeI site found at 295
  Illegal AgeI site found at 1441
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1330
  Illegal BsaI.rc site found at 194
  Illegal BsaI.rc site found at 2818